Otherwise, your experiment will not work properly since the solvent will evaporate. 3. Describe the comparisons you made. (14 points) made comparisons Of colored dyes from different sources like Reese?s Pieces and M&M Candies to determine whether the coloring components are the same. I chose the same yellow color. The independent variable I chose is the different brands. The dependent variable is measured using the chromatography paper. 4. What was your independent variable? (15 points) The independent variable I chose is the different brands. 5.
Which colors appeared to contain only one component? (15 points) The color that appears to contain only one component, based on my data, would be black. 5. Which colors were consistently composed of more than one color? (1 5 points) The color that was composed of more than one color was purple; it is made up of red and blue dye. 6. Why does the technique require you to use a pencil to mark the original spotting line? (15 points) The technique requires the use Of a pencil to mark the original spotted line because chromatography is intended to split up a suture of compounds into its subparts.
If you use a pen, the ink will also be separated. You mark it with a pencil so the mark will be there when you done with your experiment. 8. The solvent in the jar is approximately 1 CM deep, yet the spot on the paper is la CM up on the paper. Why is it important to keep the spots above the solvent? (15 points) It is important to keep the spots above the solvent because it is to protect the spots from dissolving in the solvent. If the spot is submerged in the solvent, it will go to all directions, and you can’t collect data.
Having the spot above the solvent allows for the solvent to spread up the paper by capillary action. 9. What conclusion did you reach? Were the components of the dyes similar or different? Justify your answer using your empirical evidence. (15 points) The conclusion I reached was that the components of the dyes are different. The RFC value would not be the same for every solvent as there are factors that allow each solvent to be unique. The attractive force, particle size and solubility of each solvent will create different results each time. 10.
You run a chromatograph of Non different food colorings, each consisting of a single substance. One food color (spot X) has a RFC value of 0. 350 and the other (spot Y) has a RFC value of 0. 750. Draw a sketch of the chromatograph. Include and label in your sketch the origin, the solvent front, and two labeled spots. (20 points) Origin is dotted line. Black lines are solvent fronts. Red line is spot Y. And green line is spot X. 1 1 -As the owner of a patented ink for pens, you suspect that another company has stolen the mixture. How could you use chromatography to rove they are using the same formula? 20 points) To prove another pen company has stolen the ink mixture I would use chromatography to calculate RFC=distance component moves/distance solvent moves. If I get the same RFC, then another company stole the mixture. 12. Forensic scientists use chromatography in crime scene investigations. Give an example Of an instance when a forensic scientist would need to use chromatography. (20 point s) An example of how Forensic scientists use chromatography would be in analyzing documents that are under investigation for possible forgery.
Items such as checks could be analyzed with chromatography to conclude if different inks were used while writing or signing the document in question. Submission Complete the above questions in a Word document and email the lab to your instructor via ROI Mail by the due date indicated in the Course Calendar under the Announcements Section. You can also submit your assignment by faxing it directly to your instructor. This is an option for those assignments that are not submitted electronically. Please check with your instructor for his or her specific fax number.